Volume 11, Issue 2 (2022)
JFST 2022, 11(2): 106-122 |
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Ramezanzade L, Hosseini S F, Akbari-Adergani B, Hasan Sajedi R, Yaghmur A. Evaluation of bioactive properties of peptide fractions from enzymatic hydrolysis of orangefin ponyfish (Leiognathus bindus). JFST 2022; 11 (2) : 1
URL: http://jfst.modares.ac.ir/article-6-60746-en.html
URL: http://jfst.modares.ac.ir/article-6-60746-en.html
Leila Ramezanzade1 
, Seyed Fakhreddin Hosseini * 2, Behrouz Akbari-Adergani3 , Reza Hasan Sajedi4 , Anan Yaghmur5
, Seyed Fakhreddin Hosseini * 2, Behrouz Akbari-Adergani3 , Reza Hasan Sajedi4 , Anan Yaghmur5
1- Department of Seafood Processing, Faculty of Marine Sciences, Tarbiat Modares University, Noor, Iran.
2- Department of Seafood Processing, Faculty of Marine Sciences, Tarbiat Modares University, Noor, Iran. , hosseinisf@gmail.com
3- Food & Drug Laboratory Research Center, Food & Drug Department, Ministry of Health and Medical Education, Tehran, Iran.
4- Department of Biochemistry, Faculty of biological Sciences, Tarbiat Modares University, Tehran, Iran.
5- Department of Pharmacy, University of Copenhagen, Copenhagen, Denmark.
2- Department of Seafood Processing, Faculty of Marine Sciences, Tarbiat Modares University, Noor, Iran. , hosseinisf@gmail.com
3- Food & Drug Laboratory Research Center, Food & Drug Department, Ministry of Health and Medical Education, Tehran, Iran.
4- Department of Biochemistry, Faculty of biological Sciences, Tarbiat Modares University, Tehran, Iran.
5- Department of Pharmacy, University of Copenhagen, Copenhagen, Denmark.
Abstract: (1430 Views)
In this study, the orangefin ponyfish (Leiognathus bindus) was hydrolyzed by alcalase in an enzyme to substrate ratio of 1: 100 for 300 minutes, and the degree of hydrolysis was measured for 5 hours. Also, the hydrolysate was fractionated by centrifugal having molecular mass cutoffs of 3, 10, and 30 kDa, and four peptide fractions were obtained. Then, the antioxidant activity (DPPH and ABTS free radicals scavenging activity) of peptide fractions, as well as hydrolysate, were measured at different hydrolysis times. The degree of hydrolysis was the highest (55.43 ± 2.11%) at a hydrolysis time of 240 minutes. The hydrolysate had a high amount of hydrophobic amino acids (50.6%) which cause antioxidant properties. The results of DPPH radical scavenging activity showed that the highest scavenging activity was obtained at a hydrolysis time of 240 minutes (75.59 ± 1.46). Also, among all the fractions, the 3-10 kDa fraction exhibited the highest scavenging activity compared to other fractions (80.58 ± 2.96% at a concentration of 5mg /ml). Based on the result of ABTS radical scavenging, the highest activity was reported at 240 minutes after hydrolysis (50.54 ± 0.63). Also, among all peptide fractions, the 3-10 kDa fraction had significantly higher scavenging activity than other fractions (84.58 ± 0.44 at a concentration of 5 mg /ml). The results of this study showed that the peptides obtained by enzymatic hydrolysis of orangefin ponyfish are a good candidate for providing antioxidant properties.
Article number: 1
Article Type: Original Research |
Subject:
Bioactive compounds
Received: 2022/04/10 | Published: 2022/05/31
Received: 2022/04/10 | Published: 2022/05/31
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