Volume 11, Issue 4 (2022)                   JFST 2022, 11(4): 331-345 | Back to browse issues page

‎ 20.1001.1.23225513.1401.11.4.3.6

XML Persian Abstract Print

Evaluation of stepwise precipitation of fucoidan from brown seaweed Nizamuddinia zanardinii on its chemical, molecular and antioxidant properties
Masoumeh Ghanbari Kiasara1 , Mehdi Tabarsa * 2, Hassan Ahmadi1 , Amin Mokhlesi3
1- Tarbiat Modares University, Tehran, Iran
2- Tarbiat Modares University , m.tabarsa@modares.ac.ir
3- Institute of Pharmaceutical Biology and Biotechnology, Heinrich Heine University, Dusseldorf, Germany
Abstract:   (698 Views)
The aim of the current study was the evaluation of stepwise recovery of fucoidan extracted from seaweed Nizamuddinia zanardinii and the assessment of relationship between sulfate content and molecular weight with its antioxidant activity. After the removal of pigments and low molecular weight compounds, fucoidan extracted with water was precipitated using different concentrations of ethanol at three levels (30, 50 and 70%). Three fractions of Fuc30, Fuc50 and Fuc70 showed molecular weights of 731.62, 655.76 and 10.41 × 103 g/mol, respectively. The content of sulfate esters in the obtained fractions were measured between 6.49-25.03%. The color evaluation of produced fucoidans by a digital chroma meter indicated the highest lightness index for Fuc50 fraction. Antioxidant results were varying in DPPH radical (43.93-89.40%) and ABTS radical (25.05-96.10%) scavenging activities as well as Fe3+ reducing power (Abs of 0.142-0.259). Overall, the findings of the present study indicated that stepwise precipitation of fucoidan from N. zanardinii leads to production of fractions with different chemical and molecular weight characteristics and antioxidant activities. Besides, although all isolated fucoidan fractions are introduced as potent antioxidant compounds, polymers possessing lower molecular weight and greater sulfate content exert higher antioxidant activities.
 
Article number: 3
Keywords: Fucoidan, Nizamuddinia zanardinii, Extraction, Molecular weight, Antioxidant
Full-Text [PDF 1429 kb]   (229 Downloads)    
Article Type: Original Research | Subject: Bioactive compounds
Received: 2022/08/1 | Published: 2023/02/3
Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.