Volume 4, Issue 4 (2016)                   JFST 2016, 4(4): 51-63 | Back to browse issues page

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Zamani A. The effect of some chemical factors on serinoproteinases activity of trypsin and chymotrypsin in Caspian brown trout (Salmo trutta caspius) fry. JFST 2016; 4 (4) :51-63
URL: http://jfst.modares.ac.ir/article-6-123-en.html
Head of Fisheries Department of Malayer University
Abstract:   (10380 Views)
The effect of metal ions, surfactant, oxidizing agents and enzyme inhibitors was considered on trypsin and chymotrypsin activity of the Caspian brown trout fry. The results showed K+ and Na+ didn’t significanly decrease trypsin and chymotrypsin activity (p>0.05). Ca2+ and Mg2+ significantly increased trypsin and chymotrypsin activity (p<0.05). Mn2+,Cu2+,Ba2+,Co2+,Zn2+,Fe2+ and Al3+ significantly decreased the activity of trypsin and chymotrypsin (p<0.05). Saponin and taurocholic acid significantly increased trypsin and chymotrypsin activity. Sodium cholate significantly increased chymotrypsin activity (p<0.05), but not the trypsin activity (p>0.05). Oxidizing agents, including hydrogen peroxide and sodium perborate, significantly decreased trypsin (p<0.05). Trypsin and chymotrypsin activity significantly decreased in the presence of SBTI, PMSF and ρ-Aminobenzamidine inhibitors (p>0.05). The inhibitors such as TPCK, pepstatinA, iodoacetic acid, EDTA and ß-mercaptoethanol did not significantly decrease the trypsin activity (p>0.05), but they significantly decreased chymotrypsin activity (p<0.05). Trypsin activity in the presence of TLCK showed a significant decrease (p<0.05), but  TLCK and pepstatin A  had no significant effect on chymotrypsin activity (p>0.05). 
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Article Type: Research Article | Subject: Biology and physiology
Received: 2015/08/27 | Published: 2016/02/20

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