Volume 7, Issue 3 (2018)
JFST 2018, 7(3): 185-189 |
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Rezaei Aminlooi V, Ahmadifard N, Tukmechi A, Agh N. Investigation of Microbiota of Artemia franciscana Fed by Bacterial Probiotic Bacillus subtilis and Yeast Saccharomyces Cerevisiae under Laboratory Conditions. JFST 2018; 7 (3) :185-189
URL: http://jfst.modares.ac.ir/article-6-14980-en.html
URL: http://jfst.modares.ac.ir/article-6-14980-en.html
1- Fisheries Department, Natural Resources Faculty, Urmia University, Urmia, Iran
2- Fisheries Department, Natural Resources Faculty, Urmia University, Urmia, Iran ,N.ahmadifard@urmia.ac.ir
3- Microbiology Department, Veterinary Medicine Faculty, Urmia University, Iran
4- Artemia & Aquaculture Institute, Urmia University, Urmia, Iran
2- Fisheries Department, Natural Resources Faculty, Urmia University, Urmia, Iran ,
3- Microbiology Department, Veterinary Medicine Faculty, Urmia University, Iran
4- Artemia & Aquaculture Institute, Urmia University, Urmia, Iran
Abstract: (11455 Views)
Aims: Artemia is one of the most important live foods in aquaculture. The aim of the current study was to investigate Microbiota of Artemia franciscana fed by bacterial probiotic Bacillus subtilis and yeast Saccharomyces cerevisiae under laboratory conditions.
Materials and Methods: In this experimental research, 500 Artemia nauplius were transported to 1 liter Zug with salinity of 70ppt and fed daily by Dunaliella tertiolecta and wheat bran. The present study was carried out in 4 treatments, including control treatment (no additive probiotics), probiotic treatment of Saccharomyces cerevisiae, probiotic treatment of Bacillus subtilis, and equal combination of yeast and bacteria. Statistical tests including Duncan's new multiple range test and one-way analysis of variance were used. SPSS 21 and Excel 2013 software were also used.
Findings: Bacterial probiotic treatment showed the highest colony count compared with other treatments (p<0.05). Treatment containing equal combination of yeast and bacteria had the highest colony after probiotic bacterial treatment (p<0.05). The highest number of Bacillus colonies in the body was obtained in probiotic bacterial treatment and combination of yeast and bacteria, respectively, which was significantly higher than control and yeast treatments (p<0.05). Based on the total number of Bacillus colonies, probiotic bacterial treatment showed a significant difference with other treatments and the lowest number of colony was related to probiotic yeast treatment (p<0.05).
Conclusion: Compared with other treatments, the highest number of lactic acidic bacteria is in bacterial treatment. These probiotics can well place on the surface as well as inside the body of Artemia. Therefore, using Bacillus subtilis alone or with Saccharomyces cerevisiae can have a positive effect on the bacterial count of Artemia
Materials and Methods: In this experimental research, 500 Artemia nauplius were transported to 1 liter Zug with salinity of 70ppt and fed daily by Dunaliella tertiolecta and wheat bran. The present study was carried out in 4 treatments, including control treatment (no additive probiotics), probiotic treatment of Saccharomyces cerevisiae, probiotic treatment of Bacillus subtilis, and equal combination of yeast and bacteria. Statistical tests including Duncan's new multiple range test and one-way analysis of variance were used. SPSS 21 and Excel 2013 software were also used.
Findings: Bacterial probiotic treatment showed the highest colony count compared with other treatments (p<0.05). Treatment containing equal combination of yeast and bacteria had the highest colony after probiotic bacterial treatment (p<0.05). The highest number of Bacillus colonies in the body was obtained in probiotic bacterial treatment and combination of yeast and bacteria, respectively, which was significantly higher than control and yeast treatments (p<0.05). Based on the total number of Bacillus colonies, probiotic bacterial treatment showed a significant difference with other treatments and the lowest number of colony was related to probiotic yeast treatment (p<0.05).
Conclusion: Compared with other treatments, the highest number of lactic acidic bacteria is in bacterial treatment. These probiotics can well place on the surface as well as inside the body of Artemia. Therefore, using Bacillus subtilis alone or with Saccharomyces cerevisiae can have a positive effect on the bacterial count of Artemia
Article Type: Short Communication Article |
Subject:
fish and shellfish physiology
Received: 2016/05/16 | Published: 2018/09/22
Received: 2016/05/16 | Published: 2018/09/22
References
1. Sorgeloos P. Manual for the culture and use of Brine Shrimp Artemia in aquaculture. Ghent: State University of Ghent, Faculty of Agriculture; 1986. p. 319. [Link]
2. Verschuere L, Rombaut G, Huys G, Dhont J, Sorgeloos P, Verstraete W. Microbial control of the culture of Artemia juveniles through preemptive colonization by selected bacterial strains. Appl Environl Microbiol. 1999;65(6):2527-33. [Link]
3. Shoja B, Ahmadi AR, Rafiee F, Nejatkhand Manavi P. Influence of probiotic yeast phaffia rhodozyma on growth, survival and maturity of Artemia urmiana. Asian J Experiment Biol Sci. 2012;3(2):355-9. [Link]
4. Vadstein O. The use of immunostimulation in marine larviculture: Possibilities and challenges. Aquaculture. 1997;155(1-4):401-17. [Link] [DOI:10.1016/S0044-8486(97)00114-2]
5. Cavalier-Smith T. 2003. Protist phylogeny and the high-level classification of Protozoa. Eur J Protistol. 2003;39(4):338-48. [Link] [DOI:10.1078/0932-4739-00002]
6. Ahmadvand S, Jafaryan H, Farahani A, Ahmadvand Sh. Effect of Frozen Daphnia magna diet mixed with probiotic protexin on growth and survival of Rainbow Trout (Onchorhynchus mykiss) fry reared under controlled condition. Online J Anim Feed Res. 2012;2(1):34-9. [Link]
7. Fooks LJ, Gibson GR. Probiotics as modulators of the gut flora. Br J Nutr. 2002;88 Suppl 1:S39-49. [Link] [DOI:10.1079/BJN2002628]
8. Merrifield DL, Dimitroglou A, Foey A, Davies SJ, Baker RTM, Bøgwald J, et al. The current status and future facus of probiotic and prebiotic applications for salmonids. Aquaculture. 2010;302(1-2):1-18. [Link] [DOI:10.1016/j.aquaculture.2010.02.007]
9. Dimitroglou A, Merrifield DL, Carnevali O, Picchietti S, Avella M, Daniels C, et al. Microbial Manipulations to improve fish health and production--a Mediterranean perspective. Fish and Shellfish Immunology. 2011;30(1):1-16. [Link] [DOI:10.1016/j.fsi.2010.08.009]
10. Hoseinifar SH, Mirvaghefi A, MojaziAmiri B, Khoshbavar Rostami H, Merrifield, DL. The effects of oligofructose on growth performance, survival and autochthonous intestinal microbiota of beluga (Huso huso) juveniles. Aquac Nutr. 2011;17(5):498-504. [Link] [DOI:10.1111/j.1365-2095.2010.00828.x]
11. Gomez-Gil B, Herrera-Vega MA, Aberu-Grobis FA, Roque A. Bioencapsulation of two different vibrio species in nauplii of the Brine Shrimp (Artemia franciscana). Appl Environ Microbiol. 1998;64(6):2318-22. [Link]
12. Tukmechi A, Rahmati Andani H, Manaffar R, Sheikhzadeh N. Dietary administration of beta-mercapto-ethanol treated Saccharomyces cerevisiae enhanced the growth, innate immune response and disease resistance of the rainbow trout, Oncorhynchus mykiss. Fish Shellfish Immunol. 2011;30(3):923-30. [Link] [DOI:10.1016/j.fsi.2011.01.016]
13. Coutteau P, Lavens P, Sorgeloos P. Baker's yeast as a potential substitute for live algae in aquaculture diets: Artemia as a case study. J World Aquac Soc. 1990;21(1):1-9. [Link] [DOI:10.1111/j.1749-7345.1990.tb00947.x]
14. Niu Y, Defoirdt T, Baruah K, Van De Wiele T, Dong S, Bossier P. Bacillus sp. LT3 improves the survival of gnotobiotic brine shrimp (Artemia franciscana) larvae challenged with Vibrio campbellii by enhancing the innate immune response and by decreasing the activity of shrimp-associated vibrios. Vet Microbiol. 2014;173(3-4):279-88. [Link] [DOI:10.1016/j.vetmic.2014.08.007]
15. Mahious AS, Gatesoup FJ, Hervi M, Metailler R, Ollevier F. Effect of dietary inulin and oligosaccharides as prebiotics for weaning turbot, Psetta maxima (Linnaeus, C. 1758). Aquac Int. 2006;14:219-29. [Link] [DOI:10.1007/s10499-005-9003-4]
16. Ghosh SA, Sinha A, Sabu C. Effect of probiotic on reproductive performance in female livebearing ornamental fish. Aquac Res. 2007;38(5):518-26. [Link] [DOI:10.1111/j.1365-2109.2007.01696.x]
17. Jenkins DJA, Kendall CWC, Vuksan V. Inuline, oligofructose and intestinal function. J Nutr. 1999;129(7): 1431S-3S. [Link] [DOI:10.1093/jn/129.7.1431S]
18. Tacon AGJ, Food and Agriculture Organization of the United Nations. Feed ingredients for carnivorous fish species: Alternatives to fishmeal and other fishery resources. Rome: Food and Agriculture Organization of the United Nations; 1994. [Link]
19. Zinser E, Daum G. Isolation and biochemical characterization of organelles from the yeast, Saccharomyces cerevisiae. Yeast. 1995;11(6):493-536. [Link] [DOI:10.1002/yea.320110602]
20. Ahmadnia Motlagh HR, Farhangi M, Rafiee G, Noori F. Effects of different level administration of Bacillus subtilis and Bacillus licheniformis on growth performance and survival rate of Artemia urmiana. J Fisheries Iran J Natur Resour. 2013;65(4):353-64. [Persian] [Link]
21. Vazquez JA, Gonzalez MP. Murado MA, Effects of lactic acid bacteria cultures on pathogenic microbiota from fish. Aquac. 2005;245(1-4):149-61. [Link] [DOI:10.1016/j.aquaculture.2004.12.008]
22. Ringø E, Bendiksen HR, Gausen SJ, Sundsfjord A, Olsen RE. The effect of dietary fatty acids on lactic acid bacteria associated with the epithelial mucosa and from feacalia of Arctic charr, Salvelinus alpinus (L.). J Appl Microbiol. 1998;85(5):855-67. [Link] [DOI:10.1046/j.1365-2672.1998.00595.x]
23. Patra SK, Mohamed KS. Enrichment of Artemia nauplii with the probiotic yeast Saccharomyces boulardii and its resistance against a pathogenic Vibrio. Aquac Int. 2003;11(5):505-14. [Link] [DOI:10.1023/B:AQUI.0000004193.40039.54]
24. Zorriehzahra MJ, Delshad ST, Adel M, Tiwari R, Karthik K, Dhama K, et al. Probiotics as beneficial microbes in aquaculture: An update on their multiple modes of action: A review. Vet Quart, 2016;36(4), 228-41. [Link] [DOI:10.1080/01652176.2016.1172132]
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