Effects of mucal proteins of sea anemone, Stichodactylahaddoni,on embryonic development of zebra fish, Daniorerio

Hamedi Shahraki, Maryam; Seifabadi, Seyed Jafar; Hemmati, Shahla; Khodabandeh, Saber; Shabani Panbeh Choleh, Sahar

Effects of mucal proteins of sea anemone, Stichodactylahaddoni,on embryonic development of zebra fish, Daniorerio

Authors

maryam hamedi shahraki

seyed jafar seifabadi

shahla hemmati

saber khodabandeh

sahar shabani panbeh choleh

tarbiyat modares university

Abstract

The effects of mucal proteins of sea anemone, Stichodactylahaddoni,on different stages of embryonic development of zebra fish (D. rerio) were examined. The sea anemone samples were collected from the intertidal areas of the Hormuz Island (Persian Gulf), and were frozen at -160 °C. Protein and peptide components were extracted by 100% methanol. Following the total protein assessment by ELISA, three concentrations (2.1, 3.7 and 7.4 mg/ml distil water) were prepared. From each concentration, 2 ml was added to the microplates containing 150 zebra fish eggs each, with 2 replications; microplates with normal aquarium water was also used as control group. The eggs were incubated for 72 hrs and the process of embryonic development was observed every 6 to 12 hours. Results showed that the embryonic development was normal in the control group, while the eggs treated with 3.7 and 7.4 mg/ml ofmucal proteins degenerated and blackened in less than 12 hours. Also a delay in the phase of growth in embryonic development was observed in the group with 1.2 mg/ml of protein. Our results showed that the mucal proteins from this sea anemone can affect embryonic development rapidly, causing delayed growth at low concentration, and cell lysis and embryonic degeneration at high concentrations.

Keywords

Protein compounds

mucus

Sea carpet

Persian Gulf

Embryonic development