Journal of Fisheries Science and Technology

Journal of Fisheries Science and Technology

Effect of different temperature, salinity and C/N ratio levels on biofloc production, proximate analyses and control of nitrogenous components in aquaculture system

Document Type : Original Research

Authors
Habib Sarsangi Aliabad 1
Abolfazl Naji 2
Seyed reza Seyed mortezaei 3
Iman sourinejad 2
Arash Akbarzadeh 2
1 Ph.D. student in Fisheries, Department of Fisheries, Faculty of Marine Science and Technology, University of Hormozgan, Bandar Abbas, Iran.
2 Associate Professor in Department of Fisheries, Faculty of Marine Science and Technology, University of Hormozgan, Bandar Abbas, Iran.
3 Assistant Professor in Iranian Fisheries Science Research Institute, Agricultural Research Education and Extension Organization (AREEO), Tehran, Iran.
Abstract
Biofloc technology has mentioned as a new tool for sustainable aquaculture development and has overcome the problems of water scarcity and discharge of aquaculture effluents to the environment. In this system, nitrogenous wastes (Ammonia and Ammonium) are simultaneously recovered by bacteria and converted into microbial proteins that can be consumed by aquatic animals. The purpose of this study was to investigate the factors affecting the production of biofloc and evaluate it for use in aquaculture. The effect of different temperature levels (24, 28, 32 °C), salinity (0, 4, 8 g/l) ,and C/N ratio (10:1, 15:1, 20:1) which are the main key factors to the formation and function of biofloc system was evaluated by using a response surface method designing. Moreover, the influence of those factors on total ammonia nitrogen, nitrite, nitrate, floc volume, protein, and lipid content of biofloc was investigated. The results showed that the temperature had a significant effect on floc volume and protein content of biofloc (P < 0.05), but it had no significant effect on other nitrogenous compounds and lipid content (p > 0.05). The protein, lipid and moisture of biofloc particles were decreased by increasing salinity significantly (P < 0.05). Also, TAN and nitrite concentration influenced by C/N ratio inversely. According to the factors Optimization, providing 27 °C and C/N ratio of 18:1 in brackish water and 29 °C and C/N ratio of 14:1 in fresh water resulted in high quality biofloc production and control of nitrogenous wastes in water.
Keywords
Temperature
Salinity
C/N ratio
Biofloc

Subjects

1- Ekasari J, Maryam S. Evaluation of biofloc technology application on water quality and production performance of red tilapia Oreochromis sp. cultured at different stocking densities. Hayati journal of Biosciences. 2012; 19(2):73-80.
2- Ambulkar AR. Nutrient pollution and wastewater treatment systems. InOxford Research Encyclopedia of Environmental Science 2017; 26.
3- Hargreaves JA. Biofloc production systems for aquaculture. Stoneville, MS: Southern Regional Aquaculture Center; 2013 Apr.
4- Avnimelech Y. Feeding with microbial flocs by tilapia in minimal discharge bio-flocs technology ponds. Aquaculture. 2007; 264(1-4):140-7.
5- Avnimelech Y. Carbon/nitrogen ratio as a control element in aquaculture systems. Aquaculture. 1999; 176(3-4):227-35.
6- Khanjani MH, Sajadi MM, Alizadeh M, Surinejad I. Production and evaluation of biofloc for usage in zero-exchange water system. Journal of aquaculture development. 2015; 10(1):33-42. [Persian]
7- Minabi Kh, surinejad I, Alizadeh M, Rajabzadeh A. Effect of using different C/N ratio in biofloc system on growth performance, feeding and water quality indices of common corp(Cyprinus carpio) culture. Iranian Scientific Fisheries Journal. 2018; 28(6):13-25.[Persian]
8- Becerra-Dorame MJ, Martínez-Porchas M, Martínez-Córdova LR, Rivas-Vega ME, Lopez-Elias JA, Porchas-Cornejo MA. Production response and digestive enzymatic activity of the Pacific white shrimp Litopenaeus vannamei (Boone, 1931) intensively pregrown in microbial heterotrophic and autotrophic-based systems. The Scientific World Journal. 2012 Jan 1;2012.
9- Serra FP, Gaona CA, Furtado PS, Poersch LH, Wasielesky W. Use of different carbon sources for the biofloc system adopted during the nursery and grow-out culture of Litopenaeus vannamei. Aquaculture International. 2015 Dec 1;23(6):1325-39.
10- Gao L, Shan HW, Zhang TW, Bao WY, Ma S. Effects of carbohydrate addition on Litopenaeus vannamei intensive culture in a zero-water exchange system. Aquaculture. 2012 Apr 15;342:89-96.
11- Khanjani MH. Effect of different salinity level and carbon sources in biofloc production system. Iranian Scientific Fisheries Journal. 2018; 28(4):69-79.[Persian]
12- Wilen BM, Nielsen JL, Keiding K, Nielsen PH. Influence of microbial activity on the stability of activated sludge flocs. Colloids and Surfaces B: Biointerfaces. 2000 Aug 1;18(2):145-56.
13- Luvuyo N, Nwodo UU, Mabinya LV, Okoh AI. Studies on bioflocculant production by a mixed culture of Methylobacterium sp. Obi and Actinobacteriumsp. Mayor. BMC biotechnology. 2013 Dec 1;13(1):62.
14- Xu WJ, Morris TC, Samocha TM. Effects of C/N ratio on biofloc development, water quality, and performance of Litopenaeus vannamei juveniles in a biofloc-based, high-density, zero-exchange, outdoor tank system. Aquaculture. 2016 Feb 20;453:169-75.
15- López-Elías JA, Moreno-Arias A, Miranda-Baeza A, Martínez-Córdova LR, Rivas-Vega ME, Márquez-Ríos E. Proximate composition of bioflocs in culture systems containing hybrid red tilapia fed diets with varying levels of vegetable meal inclusion. North American Journal of Aquaculture. 2015 Jan 2;77(1):102-9.
16- Association of Official Analytical Chemists. Official Methods of Analysis: Association of Official Analytical Chemists; 1990.
17- Arantes R, Schveitzer R, Seiffert WQ, Lapa KR, Vinatea L. Nutrient discharge, sludge quantity and characteristics in biofloc shrimp culture using two methods of carbohydrate fertilization. Aquacultural engineering. 2017 Jan 1;76:1-8.
18- Hostins B, Braga A, Lopes DL, Wasielesky W, Poersch LH. Effect of temperature on nursery and compensatory growth of pink shrimp Farfantepenaeus brasiliensis reared in a super-intensive biofloc system. Aquacultural Engineering. 2015 May 1;66:62-7.
19- Boyd CE. Pond water aeration systems. Aquacultural engineering. 1998 Jul 1;18(1):9-40.
20- Ponce-Palafox JT, Pavia ÁA, López DG, Arredondo-Figueroa JL, Lango-Reynoso F, del Refugio Castañeda-Chávez M, Esparza-Leal H, Ruiz-Luna A, Páez-Ozuna F, Castillo-Vargasmachuca SG, Peraza-Gómez V. Response surface analysis of temperature-salinity interaction effects on water quality, growth and survival of shrimp Penaeus vannamei postlarvae raised in biofloc intensive nursery production. Aquaculture. 2019 Mar 30;503:312-21.
21- Hargreaves JA. Photosynthetic suspended-growth systems in aquaculture. Aquacultural engineering. 2006 May 1;34(3):344-63.
22- Hakanson L. The relationship between salinity, suspended particulate matter and water clarity in aquatic systems. Ecological Research. 2006 Jan 1;21(1):75-90.
23- Maica PF, de Borba MR, Wasielesky Jr W. Effect of low salinity on microbial floc composition and performance of Litopenaeus vannamei (Boone) juveniles reared in a zero‐water‐exchange super‐intensive system. Aquaculture Research. 2012 Feb;43(3):361-70.
24- Lara G, Krummenauer D, Abreu PC, Poersch LH, Wasielesky W. The use of different aerators on Litopenaeus vannamei biofloc culture system: effects on water quality, shrimp growth and biofloc composition. Aquaculture International. 2017 Feb 1;25(1):147-62.
25- Crab R, Lambert A, Defoirdt T, Bossier P, Verstraete W. The application of bioflocs technology to protect brine shrimp (Artemia franciscana) from pathogenic Vibrio harveyi. Journal of applied microbiology. 2010 Nov;109(5):1643-9.
26- Ray AJ, Drury TH, Cecil A. Comparing clear-water RAS and biofloc systems: Shrimp (Litopenaeus vannamei) production, water quality, and biofloc nutritional contributions estimated using stable isotopes. Aquacultural Engineering. 2017 May 1;77:9-14.
27- Emerenciano M, Gaxiola G, Cuzon G. Biofloc technology (BFT): a review for aquaculture application and animal food industry. Biomass now-cultivation and utilization. 2013 Apr 30:301-28.
28- Ekasari J, Crab R, Verstraete W. Primary nutritional content of bio-flocs cultured with different organic carbon sources and salinity. HAYATI Journal of Biosciences. 2010 Sep 1;17(3):125-30.