Journal of Fisheries Science and Technology

Journal of Fisheries Science and Technology

Evaluation of killed vaccine potency against Nervous Necrosis Virus (NNV) using superoxide dismutase and lysozyme parameters of Acipenser stellatus Pallas, 1771 serum assay

Document Type : Original Research

Authors
Elahe Afsharipour 1
Ghobad Azari Takami 1
Seyed Mohammad Jalil Zorriehzahra 2
Abbas Ali Motallebi 1
Shapour Kakoolaki 3
1 Department of Health and Food, Islamic Azad University, Science and Research Branch, Tehran, Iran
2 Scientific Information and Communication Department,Iranian Fisheries Science Research Institute (IFSRI),Agricultural Research Education and Extension Organization(AREEO), Tehran, Islamic Republic of Iran
3 Department of Aquatic animal Health and Diseases, Iranian Fisheries Science Research Institute, Agricultural Research, Education and Extension Organization, Tehran, Iran
Abstract
In this study, the effectiveness of 4 killed VNN vaccines along with three types of adjuvants was evaluated by both immersion and injection methods. About 540 fish weighing 7-10 g of ozone (Acipenser stellatus Pallas 1771) were considered. Vaccination was performed in two stages one month apart, and one month after the second recurrence, exposure to the acute live virus was performed. During this period, the mortality rate of immersion and injection groups was 12.9% and 19.8%, respectively, compared to 100% mortality in the control group. Blood sampling was performed to assess immune factors (superoxide dismutase, lysozyme) in four stages before the first vaccination in the adaptation period, after the first vaccination, after the second vaccination one month and after exposure to live virus Acute was performed to identify changes before and after exposure to the virus. The results of the present study showed that immunization vaccination in the vaccinated group with the vaccine containing IMS 1312 SEPPIC adjuvant significantly higher levels of superoxide dismutase 1745 IU / MI (p<0.05) and lysozyme 40.6 (p<0.05). Compared to other groups, which proves its better efficacy compared to other vaccines. Therefore, a vaccine killed with 75% IMS 1312 SEPPIC adjuvant can be recommended for vaccination against VNN.
Keywords
Sturgeon
Nodavirous
VNN
Acipenser stellutus Pallas 1771
Vaccination
Immunization
Hematologic factors

Subjects

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