Journal of Fisheries Science and Technology

Journal of Fisheries Science and Technology

Investigation and identification of barcode of common carp Cyprinus carpio in the southern coast of the Caspian Sea

Document Type : Original Research

Authors
Mohamad Larijani 1
Roya Bakhtiar 2
Mehrnoush Norouzi 3
Raha Fadaei 4
1 Aquatic Resources Research Center
2 Department of Animal Sciences, Campus of Agriculture and Natural Resources, University of Tehran, Karaj
3 Islamic Azad University, Tonekabon Branch
4 Department of Fisheries Engineering, Faculty of Natural Resources, Jiroft University, Kerman
Abstract
This study was performed to determine the identification (barcoding) using cytochrome oxidase gene of common carp, between three provinces of Golestan, Mazandaran and Gilan (respectively in Gomishan, Tajan and Kiashahr) in 2011. The results of sequencing showed that all samples from the three regions had a genetic distance less than 2%, so all samples were from the same species. The results of sequencing 30 tail samples of carp species on the southern shores of the Caspian Sea showed that all samples are of the same species and their genetic distance does not reach at least 2%. Therefore, all carp samples of the three provinces are of the same species and have the same type of barcode. In the study of nucleotide and haplotypic distance, Gomishan region was 10.75000, 1 and Kiashahr region were 3.200 and 0.9333, respectively. In the study of nucleotide diversity between the two regions, 0.01978 and the average nucleotide difference was 12.187. Haplotypic diversity in Gomishan region was 38.095 and in Kiashahr region was 23.809%. Out of 13 haplotypes, Gomishan region with 8 haplotypes (61.53%) and Kiashahr region with 5 haplotypes (38.46%) had the lowest haplotypes.The results of this study show that there is a significant difference between carp samples in Gomishan and Kiashahr regions in terms of nucleotide and haplotypic diversity (P <0.05).

Keywords
Barcode
common carp
Nucleotide and haplotype diversity
Caspian sea

Subjects

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