Journal of Fisheries Science and Technology

Journal of Fisheries Science and Technology

Evaluation of antimicrobial activity of a combination of bacteria from farmed Pacific white shrimp Penaeus vannamei Boone, 1931, in vitro and in vivo phases

Document Type : Original Research

Authors
Saeedeh Sarhadi 1
Iman sourinezhad 1
Mohsen Gozari 2
1 University of Hormozgan
2 Persian Gulf and Oman Sea Ecology Research Center
Abstract
Evaluation of the antimicrobial activity of bacteria in the aquaculture ecosystem is the first stage of probiotic bacteria screening studies. The aim of the present study was to isolate the intestinal bacteria of cultivated Vanammei shrimp with antimicrobial activity against pathogenic bacteria in vitro and in vivo. For this purpose, sampling of shrimp culture sites in South Tiab and North Tiab was done in 3 stages. The results of counting culturable bacteria using Zobell agar culture medium showed that in South Tiab site, the average number of bacteria in shrimp intestine samples was from 3.66 × 106 CFU/gr in the first stage of sampling to 4.63 ×106 CFU/gr in the third stage. With a similar trend, the changes of this amount in the North Tiab site fluctuated from 16.4 ×106 CFU/gr to 16.16 ×106 CFU/gr. Evaluation of the antimicrobial activity of the isolates using the agar diffusion method showed that 9, 6, 4 and 3 isolates respectively compared to V. alginolyticus, V. harveyi, V. parahaemolyticus and P. aeroginosa showed antimicrobial activity.
Keywords
Intestine bacteria
Genetic identification
Microbial challenge test
Penaeus vannamei

Subjects

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