Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

---

Listeria monocytogenes is a foodborne pathogen which has high resistance for unfavorable conditions. This pathogen is able to entering Viable but non culturable (VBNC) state in harsh conditions. The present study was aimed to consider the possibility of entering this pathogen in to VBNC state in heavy brine which used for produce brined fish. For this purpose L. monocytogenesat 1.12×10⁷ initial concentration as monitored in three treatments during 8 days: brine containing 30% salt (ES30), brine containing 10% salt (ES10) and starvation condition (DE).  For considering alive cells method of gene expression of 16S rRNAby RT-PCR was used. The obtained results showed that this pathogen in ES30 and DE treatments after three days enter in to VBNC state. According to obtained results ES10 treatment entering in to VBNC state on 5 days after inoculation. The results showed that there is the possibility of L. monocytogenes entering in to VBNC state, during brined and smoked fish or every other brined food product and defect of Standard detection methods. 

Keywords: Listeria monocytogenes, Salt, Viable but non culturable (VBNC), Listeria monocytogenes, Salted fish

Full-Text [PDF 811 kb]   (2756 Downloads)