Volume 4, Issue 3 (2015)                   JFST 2015, 4(3): 47-59 | Back to browse issues page

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Jafari Taraji R, Alishahi A, Ojagh S M, Esmaeili Molla A. Protein hydrolysates from viscera of cultured Siberian sturgeon (Acipenser baerii) and its use for bacterial (Salmonella typhi) culture medium. JFST 2015; 4 (3) :47-59
URL: http://jfst.modares.ac.ir/article-6-11788-en.html
Protein hydrolysates from viscera of cultured Siberian sturgeon (Acipenser baerii) and its use for bacterial (Salmonella typhi) culture medium
Roghayeh Jafari Taraji * 1, Alireza Alishahi2 , Seyed Mahdi Ojagh3 , Abbas Esmaeili Molla4
1- Gorgan University of Agricultural Sciences and Natural Resources
2- Assistant Professor
3- Assistant Professor of Seafood Processing Department
4- Assistant Professor of Aquatic Physiology
Abstract:   (9011 Views)
Protein hydrolysate (PH) from viscera of cultured Siberian sturgeon (Acipenser baerii) was produced. To optimize the production conditions, Response Surface Method (RSM) was employed to examine the effects of three different operating conditions, including time, pH, and enzymatic concentration (Alcalase) on the degree of hydrolysis.The mathematical model showed acceptable fitness of the experimental data as R2 equaled 0.97, which indicated  that   major part of  the  variability  within  the  range  of  values could  be explained  by  the  model. The results showed that the highest degree of hydrolysis (58.21%) was related to the treatment which happened at the enzymatic concentration of 2%, 60 minutes time, and pH=8. Treatment under hydrolysis condition (i.e., E/S = 2%, Time = 45 min, and pH = 8.5) had the highest protein content (42.37g/l), which was used as an alternative to commercial peptone medium (Triptic soy broth) to assess the growth of Salmonella typhi bacteria from 0 to 48 hours. Although there was an upward trend in growth rate of S. typhi both in control and No. 15 (Alcalase) treatments, the log growth of control treatment was found to be better than that of Alcalase treatment. However, there existed no significant difference between the two treatments.
Keywords: Siberian sturgeon (Acipenser baerii), Salmonella typhi, Protein hydrolysates, Alcalase
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Article Type: Research Article | Subject: Sea food processing
Received: 2015/06/7 | Published: 2015/11/22
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