Volume 8, Issue 1 (2019)                   JFST 2019, 8(1): 23-30 | Back to browse issues page

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Salamroodi E, Rezaei Tavabe K, Javanmardi S. Effects of Different Levels of Dietary Vitamin C on Growth Performance, Digestive Enzymes Activity and Blood Stress Factors of Nile Tilapia (Oreochromis Niloticus) Under Sub-Lethal Toxicity of Malathion. JFST 2019; 8 (1) :23-30
URL: http://jfst.modares.ac.ir/article-6-27493-en.html
Effects of Different Levels of Dietary Vitamin C on Growth Performance, Digestive Enzymes Activity and Blood Stress Factors of Nile Tilapia (Oreochromis Niloticus) Under Sub-Lethal Toxicity of Malathion
E. Salamroodi1 , K. Rezaei Tavabe * 2, S. Javanmardi1
1- Fisheries Department, Natural Resources Faculty, Agriculture & Natural Resources Campus, University of Tehran, Karaj, Iran
2- Fisheries Department, Natural Resources Faculty, Agriculture & Natural Resources Campus, University of Tehran, Karaj, Iran , krtavabe@ut.ac.ir
Abstract:   (5617 Views)

Aims: Malathion is one of the agricultural organophosphate pesticides used in agriculture that may be found in aquaculture centers. The aim of this study was to investigate the effects of different levels of dietary vitamin C on improving the situation of growth, digestive enzymes activity and blood stress factors of Nile Tilapia (Oreochromis Niloticus) under sub-lethal toxicity of malathion.

Materials & Methods: In this experimental study, 180 Nile tilapia with weight average of 12.5±5g were subjected to 25% concentrations of Lc50 96h of malathion for 28 days and during this period, the fishes were fed with different levels of vitamin C (0, 300, 600, and 900mg/kg of feed). Data were analyzed by Duncan's new multiple range test and one-way analysis of variance through SPSS 24 software.

Findings: The weight gain and specific growth rate of fish fed by the highest vitamin level (900mg/kg of feed) were significantly higher than other groups (p<0.05), while the feed conversion ratio in the fish under mentioned treatment significantly decreased compared to other treatments. Trypsin and chymotrypsin digestive enzyme activities were significantly increased in treatments of 600 and 900mg/kg vitamin C in diet, respectively. The activity of other enzymes at different concentrations of vitamin C in the diet did not show a significant difference (p>0.05). Blood glucose level was significantly higher in control treatment in comparison to the other treatments.

Conclusion: Vitamin C as an anti-stress agent reduces the stress in Nile tilapia subjected by Malathion pesticides and improve the metabolic status and growth performance of Nile tilapia.

Keywords: Tilapia, Ascorbic Acid, Digestive System, Malathion, Physiological Stress
Full-Text [PDF 936 kb]   (1824 Downloads)    
Article Type: Original Research | Subject: Aquatics Biology
Received: 2017/11/24 | Published: 2019/03/19
References
1. Naji T, Khara H, Rostami M, Nasiri Parman E. Effect of ammonia toxicity on common carp liver (Cyprinus carpio). J Environ Sci Technol. 2009;11(1):131-48. [Persian] [Link]
2. Shahsavani D, Mohri M, Nazeri K. A study of effects of anionic detergent (shampoo) on blood parameters of gold fish (Carassius auratus). Pajouhesh Va Sazandegi. 2003;16(4):99-103. [Persian] [Link]
3. Ghovati N, Mohammadi S, Mohammadi V. Assess changes in hardness and alkalinity on the toxicity of zinc poisoning in common carp (Cyprinus carpio). J Wetl Ecobiol. 2011;2(8):21-8. [Persian] [Link]
4. Sinyakov MS, Dror M, Zhevelev HM, Margel Sh, Avtalion RR. Natural antibodies and their significance in active immunization and protection against a defined pathogen in fish. Vaccine. 2002;20(31-32):3668-74. [Link] [DOI:10.1016/S0264-410X(02)00379-1]
5. Baratizade S, Peyghan R, Razijalali M. Combined effect of filtration method (carbon, zeolite and simple filtration) and stock density of Macro (Labidochromis caeruleus) on growth and nitrogenous compounds of water. J Vet Med Iran. 2017;13(3):15-23. [Persian] [Link] [DOI:10.1016/S1365-6937(17)30029-1]
6. Banaee M, Sureda A, Mirvaghefi AR, Ahmadi K. Effects of diazinon on biochemical parameters of blood in rainbow trout (Oncorhynchus mykiss). Pestic Biochem Physiol. 2011;99(1):1-6. [Link] [DOI:10.1016/j.pestbp.2010.09.001]
7. Ebrahimzade MA, Karami M, Golrokh M. Measurement of choline esterase activity in the Rutilus kutum brain in marine and breeding types as an indicator of the health of these fishes. Shahrekord Univ Med Sci. 2004;6(3):33-8. [Persian] [Link]
8. Ghafari Farsani H, Poorbagher H, Farahmand H. Effects of malathion on DNA breakage in the liver and gill of rainbow trout (Oncorhynchus Mykiss) using weighted averaging. J Fish. 2016;69(1):89-99. [Persian] [Link]
9. Lu Ch, Toepel K, Irish R, Fenske RA, Barr DB, Bravo R. Organic diets significantly lower children's dietary exposure to organophosphorus pesticides. Environ Health Perspect. 2005;114(2):260-3. [Link] [DOI:10.1289/ehp.8418]
10. Yonar SM, Ural MŞ, Silici S, Yonar ME. Malathion-induced changes in the haematological profile, the immune response, and the oxidative/antioxidant status of Cyprinus carpio carpio: Protective role of propolis. Ecotoxicol Environ Saf. 2014;102(8):202-9. [Link] [DOI:10.1016/j.ecoenv.2014.01.007]
11. Kaya H, Çelik EŞ, Yılmaz S, Tulgar A, Akbulut M, Demir N. Hematological, serum biochemical, and immunological responses in common carp (Cyprinus carpio) exposed to phosalone. Comp Clin Pathol. 2015;24(3):497-507. [Link] [DOI:10.1007/s00580-014-1930-x]
12. Wahli T, Verlhac V, Girling P, Gabaudan J, Aebischer C. Influence of dietary vitamin C on the wound healing process in rainbow trout (Oncorhynchus mykiss). Aquaculture. 2003;225(1-4):371-86. [Link] [DOI:10.1016/S0044-8486(03)00302-8]
13. Sandnes K, Ulgenes Y, Braekkan OR, Utne F. The effect of ascorbic acid supplementation in broodstock feed on reproduction of rainbow trout (Salmo gairdneri). Aquaculture. 1984;43(1-3):167-77. [Link] [DOI:10.1016/0044-8486(84)90019-X]
14. Arab N, Rajabi Islami H. Effects of dietary ascorbic acid on growth performance, body composition, and some immunological parameters of Caspian brown trout, Salmo trutta caspius. J World Aquac Soc. 2015;46(5):505-18. [Link] [DOI:10.1111/jwas.12215]
15. Terova G, Saroglia M, Papp ZG, Cecchini S. Dynamics of collagen indicating amino acids, in embryos and larvae of sea bass (Dicentrarchus labrax) and gilthead sea bream (Sparus aurata), originated from broodstocks fed with different vitamin C content in the diet. Comp Biochem Physiol Part A Mol Integr Physiol. 1998;121(2):111-8. [Link] [DOI:10.1016/S1095-6433(98)10110-1]
16. Suresh AV, Lin CK. Tilapia culture in saline waters: A review. Aquaculture. 1992;106(3-4):201-26. [Link] [DOI:10.1016/0044-8486(92)90253-H]
17. Wang M, Lu M. Tilapia polyculture: A global review. Aquac Res. 2016;47(8):2363-74. [Link] [DOI:10.1111/are.12708]
18. El-Rhman AM, Khattab YA, Shalaby AM. Micrococcus luteus and Pseudomonas species as probiotics for promoting the growth performance and health of Nile tilapia, Oreochromis niloticus. Fish Shellfish Immunol. 2009;27(2):175-80. [Link] [DOI:10.1016/j.fsi.2009.03.020]
19. Pathiratne A, George SG. Toxicity of malathion to Nile tilapia, Oreochromis niloticus and modulation by other environmental contaminants. Aquat Toxicol. 1998;43(4):261-71. [Link] [DOI:10.1016/S0166-445X(98)00059-9]
20. Mohapatra S, Chakraborty T, Prusty AK, Kumar K, Prasad KP, Mohanta KN. Fenvalerate induced stress mitigation by dietary supplementation of multispecies probiotic mixture in a tropical freshwater fish, Labeo rohita (Hamilton). Pestic Biochem Physiol. 2012;104(1):28-37. [Link] [DOI:10.1016/j.pestbp.2012.06.006]
21. Gabriel UU, Ugbomeh AP. Efficacy of Clove flower bud powder as anaesthetic for three life stages of S. melanotheron, O. niloticus and T. Guineensis. J Appl Sci Environ Manag. 2017;21(1):11-5. [Link] [DOI:10.4314/jasem.v21i1.2]
22. Ricker WE. Computation and interpretation of biological statistics of fish populations. Ottawa: Fisheries Research Board of Canada; 1975. [Link]
23. Hummel BCW. A modified spectrophotometric determination of chymotrypsin, trypsin, and thrombin. Can J Biochem Physiol. 1959;37(12):1393-9. https://doi.org/10.1139/o59-157 [Link] [DOI:10.1139/y59-157]
24. Shihabi ZK, Bishop Ch. Simplified turbidimetric assay for lipase activity. Clin Chem. 1971;17(12):1150-3. [Link]
25. Bernfeld P. Enzymes of starch degradation and synthesis. Adv Enzymol Relat Areas Mol Biol. 1951;12:379-428. [Link] [DOI:10.1002/9780470122570.ch7]
26. Lee KJ, Dabrowski K. Long-term effects and interactions of dietary vitamins C and E on growth and reproduction of yellow perch, Perca flavescens. Aquaculture. 2004;230(1-4):377-89. [Link] [DOI:10.1016/S0044-8486(03)00421-6]
27. Ruff N, Fitzgerald RD, Cross TF, Hamre K, Kerry JP. The effect of dietary vitamin E and C level on market‐size turbot (Scophthalmus maximus) fillet quality. Aquac Nutr. 2003;9(2):91-103. [Link] [DOI:10.1046/j.1365-2095.2003.00230.x]
28. Sarma K, Pal AK, Sahu NP, Ayyappan S, Baruah K. Dietary high protein and vitamin C mitigates endosulfan toxicity in the spotted murrel, Channa punctatus (Bloch, 1793). Sci Total Environ. 2009;407(12):3668-73. [Link] [DOI:10.1016/j.scitotenv.2009.02.031]
29. Narra MR, Rajender K, Rudra Reddy R, Venkateswara Rao J, Begum G. The role of vitamin C as antioxidant in protection of biochemical and haematological stress induced by chlorpyrifos in freshwater fish Clarias batrachus. Chemosphere. 2015;132:172-8. [Link] [DOI:10.1016/j.chemosphere.2015.03.006]
30. Adel A, Khara H. The effects of different dietary vitamin C and iron levels on the growth, hematological and immunological parameters of rainbow trout Oncorhynchus mykiss fingerlings. Iran J Fish Sci. 2016;15(2):886-97. [Persian] [Link]
31. Javanmardi S, Rezaee Tavabe K, Moradi S, Bayat Ghyasi LS. Effects of different levels of dietary vitamin C on growth performance, digestive enzymes activity and blood stress factors of rainbow trout under sub-lethal toxicity of malathion. J Aquac Sci. 2018;5(2):40-49. [Persian] [Link]
32. Soivio A, Oikari A. Haematological effects of stress on a teleost, Esox lucius L. J Fish Biol. 1976;8(5):397-411. [Link] [DOI:10.1111/j.1095-8649.1976.tb03968.x]
33. Dogan D, Can C. Hematological, biochemical, and behavioral responses of Oncorhynchus mykiss to dimethoate. Fish Physiol Biochem. 2011;37(4):951-8. [Link] [DOI:10.1007/s10695-011-9492-1]
34. Ahmad Z. Acute toxicity and haematological changes in common carp (Cyprinus carpio) caused by diazinon exposure. Afr J Biotechnol. 2011;10(63):13852-9. [Link] [DOI:10.5897/AJB11.1247]
35. Al-Ghanim KA. Acute toxicity and effects of sub-lethal malathion exposure on biochemical and haematological parameters of Oreochromis niloticus. Sci Res Essays. 2012;7(16):1674-80. [Link] [DOI:10.5897/SRE12.039]
36. Martínez-Porchas M, Martínez-Córdova LR, Ramos-Enriquez R. Cortisol and glucose: Reliable indicators of fish stress?. Pan Am J Aquat Sci. 2009;4(2):158-78. [Link]
37. Farrell AP, editor. Encyclopedia of fish physiology: From genome to environment. 1st Edition. Cambridge: Academic Press; 2011. [Link]
38. Hamed HS. Impact of a short-term malathion exposure of Nile tilapia,(Oreochromis niloticus): The protective role of selenium. Int J Environ Monit Anal. 2015;3(5-1):30-7. [Link]
39. Abdel Gawad EA, Abdel Hamid OM. Effect of vitamin C dietary supplementation in reducing the alterations induced by fenitrothion in Oreochromis niloticus. Fish Physiol Biochem. 2014;40(3):787-96. [Link] [DOI:10.1007/s10695-013-9885-4]
40. Jaddi Y, Movahedinia A, Safahieh A, Dejandian S, Hallajian A. Sub-lethal effects of pesticide diazinon on some serum biochemical parameters of Caspian Sea common bream (Abramis brama) fingerlings. J Anim Res. 2015;28(3):274-81. [Persian] [Link]
41. Mirvaghefi A, Farahmand H, Rafiee G, Banaee M. Study of biochemical characteristics of blood and histopathology in experimental poisoning with diazinon in common carp (Cyprinus carpio). J Fish. 2012;65(2):119-33. [Persian] [Link]
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