Volume 5, Issue 1 (2016)                   JFST 2016, 5(1): 69-82 | Back to browse issues page

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Rezaei M, Khodabandeh S, Motamedzadegan A, Norozinia M. Optimization of Persian Gulf cuttlefish (Sepia pharaonis) muscle hydrolysate production by RSM. JFST 2016; 5 (1) :69-82
URL: http://jfst.modares.ac.ir/article-6-4008-en.html
Abstract:   (9066 Views)
The response surface methodology was employed to optimize the effects of pH, temperature (˚C), time (min) and the ratio of enzyme to substrate (% of substrate) on the hydrolysis process of cuttlefish muscle by alcalase. Central composite rotatable design with 5 levels and 4 factors and α=2 was used for the optimization of the process to gain the highest degree of hydrolysis. pH, temperature, time, enzyme concentration, interaction of temperature-enzyme concentration, square of pH, temperature, time and enzyme concentration had significant effects on the process. The R2 = 0.95, lack of fit < 0.05 and adeq-Precision of 14.16 for the model showed that the model could explain the variability within the range of values. The optimum condition for 42.0117 % of degree of hydrolysis was determined by Design Expert as pH 8.19, temperature 50.23, time 129.62 and enzyme2.15%.
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Article Type: Research Article | Subject: Sea food processing
Received: 2015/03/2 | Published: 2016/05/21

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