Journal of Fisheries Science and Technology

Journal of Fisheries Science and Technology

The effect of different hormonal inducing agents on blood plasma sex steroids and biochemical parameters of the Caspian roach (Rutilus caspicus) female breeders

Document Type : Original Research

Authors
Erfan Akbari Nargesi 1
Bahram Falahatkar 1
Daniel Zarski 2
Danial Gorouhi 3
1 University of Guilan
2 Institute of Animal Reproduction and Food Research, Polish Academy of Sciences
3 Shahid Ansari Teleost Fish Restocking and Genetic Conservation Center
Abstract
The present study aimed to investigate the effects of injecting different hormonal agents on sex steroids levels and biochemical parameters of the blood plasma in females Caspian roach (Rutilus caspicus). In order to perform the experiment, groups of five fish were injected intraperitoneally as follows: 1) two injections of Ovaprim (Ova), 2) two injections of Ovopel (Ovo), 3) a priming dose of Ovopel with a resolving dose of Ovaprim (Comb1), 4) a priming dose of Ovaprim with a resolving dose of Ovopel (Comb2), and 5) sterile 0.9% saline. According to the observations, the highest concentrations of progesterone and estradiol were measured in control group and concentrations of these hormones sharply declined after spawning in the experimental treatments (P < 0.05). Moreover, the lowest testosterone concentration was observed in the control group and after spawning, its concentration showed a significant increase in hormone-induced treatments (P < 0.05). The highest plasma glucose concentration was obtained in the control group, and the lowest concentration was observed in the Comb1 treatment (P < 0.05). No significant difference was observed between experimental treatments in the concentration of plasma biochemical parameters (P > 0.05). Overall, the present study showed that hormonal agents can significantly affect the levels of sex steroids in the blood plasma of Caspian roach breeders. These results showed the need to consider the proper induction strategies and welfare conditions of Caspian roach breeders in captivity.
Keywords
Broodstock management
Cyprinidae
Physiological parameters
reproduction

Subjects

[1] Seibel, H., Baßmann, B., Rebl, A., 2021. Blood will tell: what hematological analyses can reveal about fish welfare. Frontiers in Veterinary Science, 8, p.616955.
[2] Witeska, M., Kondera, E., Ługowska, K., Bojarski, B., 2022. Hematological methods in fish–Not only for beginners. Aquaculture, 547, p.737498.
[3] Fazio, F., 2019. Fish hematology analysis as an important tool of aquaculture: a review. Aquaculture, 500, pp.237-242.
[4] Akbari Nargesi, E., Falahatkar, B., Sajjadi, M.M., 2020. Dietary supplementation of probiotics and influence on feed efficiency, growth parameters and reproductive performance in female rainbow trout (Oncorhynchus mykiss) broodstock. Aquaculture Nutrition, 26, 98–108.
[5] Podhorec, P., Kouril, J., 2009. Induction of final oocyte maturation in Cyprinidae fish by hypothalamic factors: A review. Veterinarni Medicina, 54, 97–110.
[6] Naddafi, R., Abdoli, A., Kiabi, B.H., Amiri, B.M., Karami, M., 2005. Age, growth and reproduction of the Caspian roach (Rutilus rutilus caspicus) in the Anzali and Gomishan wetlands, North Iran. Journal of Applied Ichthyology, 21, 492–497.
[7] Nelson, J.S., Grande, T.C., Wilson, M.V.H., 2016. Fish of the World. 5th Edition, John Wiley and Sons, Hoboken. 752 p.
[8] Babin, P.J., Cerdà, J., Lubzens, E., 2007. Fish Oocyte: From Basic Studies to Biotechnological Applications. Springer, Netherlands. 508 p.
[9] Mylonas, C.C., Fostier, A., Zanuy, S., 2010. Broodstock management and hormonal manipulations of fish reproduction. General and Comparative Endocrinology, 165, 516–534.
[10] Akbari Nargesi, E., Falahatkar, B., Żarski, D., Gorouhi, D., 2023. The effectiveness of Ovaprim, Ovopel, and their combinations in artificial reproduction of common rudd Scardinius erythrophthalmus under controlled conditions. Theriogenology, 199, pp.114-120.
[11] Lubzens, E., Young, G., Bobe, J., Cerdà, J., 2010. Oogenesis in teleosts: How fish eggs are formed. General and Comparative Endocrinology, 165, 367–389.
[12] Zohar, Y., Muñoz-Cueto, J.A., Elizur, A., Kah, O., 2010. Neuroendocrinology of reproduction in teleost fish. General and Comparative Endocrinology, 165, 438–455.
[13] Akbari Nargesi, E., Falahatkar, B., Żarski, D., 2022. Artificial reproduction of Caspian roach, Rutilus caspicus following stimulating ovulation with Ovaprim, Ovopel, and their combinations under controlled conditions. Animal Reproduction Science, 238, p.106932.
[14] Akbari Nargesi, E., 2022. Reproductive performance and physiological changes of the Caspian roach, Rutilus caspicus broodstocks under controlled conditions after induction with Ovopel and Ovaprim. PhD thesis. University of Guilan. 119 p.
[15] Jamróz, M., Kucharczyk, D., Hakć-Błażowska, A., Krejszeff, S., Kujawa, R., Kupren, K., Kwiatkowski, M., Targońska, K., Żarski, D., Cejko, B.I., Glogowski, J., 2008. Comparing the effectiveness of Ovopel, Ovaprim, and LHRH analogue used in the controlled reproduction of ide, leuciscus idus (L.). Archives of Polish Fisheries, 16, 363–370.
[16] Żarski, D., Kucharczyk, D., Targońska, K., Jamróz, M., Krejszeff, S., Mamcarz, A., 2009. Application of Ovopel and Ovaprim and their combinations in controlled reproduction of two reophilic cyprinid fish species. Polish Journal of Natural Sciences, 4, 235-244.
[17] Targońska, K., Kupren, K., Kujawa, R., Mamcarz, A., Kaczkowski, Z., Glogowski, J., Kowalski, R.K., Żarski, D., Wyszomirska, E., Kucharczyk, D., 2015. Artificial reproduction of different dace, Leuciscus leuciscus (L.) populations as a method for biodiversity preservation. Turkish Journal of Fisheries and Aquatic Sciences, 15, 477–485.
[18] Hakuć-Błażowska, A., Kupren, K., Turkowski, K., Targońska, K., Żarski, D., Kucharczyk, D., 2010. A comparison of the economic effectiveness of various spawning agents for stimulating the reproduction of the cultured and wild forms of the common barbel Barbus barbus (L.). Polish Journal of Natural Sciences, 25, 272–286.
[19] Kucharczyk, D., Nowosad, J., Wyszomirska, E., Cejko, B.I., Arciuch-Rutkowska, M., Juchno, D., Boroń, A., 2020. Comparison of artificial spawning effectiveness of hCG, CPH and GnRHa in combination with dopamine inhibitors in a wild strain of ide Leuciscus idus (L.) in hatchery conditions. Animal Reproduction Science, 221, 106543.
[20] Campbell, T.W., 2015. Exotic Animal Hematology and Cytology: Fourth Edition. Wiley Blackwell, New York, United States. 402 p.
[21] Nagahama, Y., 2002. Endocrine regulation of gametogenesis in fish. International Journal of Developmental Biology, 38, 217–229.
[22] Nagahama, Y., Yamashita, M., 2008. Regulation of oocyte maturation in fish. Development, Growth and Differentiation, 50, 195–219.
[23] Johnson, K., Thomas, P., Wilson, R.R., 1998. Seasonal cycles of gonadal development and plasma sex steroid levels in Epinephelus morio, a protogynous grouper in the eastern Gulf of Mexico. Journal of Fish Biology, 52, 502–518.
[24] Poortenaar, C.W., Hooker, S.H., Sharp, N., 2001. Assessment of yellowtail kingfish (Seriola lalandi lalandi) reproductive physiology, as a basis for aquaculture development. Aquaculture, 201, 271–286.
[25] Lee, W.K., Yang, S.W., 2002. Relationship between ovarian development and serum levels of gonadal steroid hormones, and induction of oocyte maturation and ovulation in the cultured female Korean spotted sea bass Lateolabrax maculatus. Aquaculture, 207, 169–183.
[26] Heidari, B., Roozati, S., 2018. Changes in plasma levels of steroid hormones during oocyte development of Caspian kutum (Rutilus frisii kutum, Kamensky, 1901). Animal Reproduction, 7, 373–381.
[27] Falahatkar, B., Poursaeid, S., Ershad Langroudi, H., Efatpanah, I., Meknatkhah, B., Rahmati, M., 2013. Spawning induction in kutum, Rutilus frisii kutum (Kamensky), with different hormones: Analysis of hormone profiles and induced spawning success. Fisheries and Aquatic Life, 21, 271–281.
[28] Akhoundian, M., Salamat, N., Savari, A., Movahedinia, A., Salari, M.A., 2020. Influence of photoperiod and temperature manipulation on gonadal development and spawning in Caspian roach (Rutilus rutilus caspicus): Implications for artificial propagation. Aquaculture Research, 51, 1623–1642.
[29] Kobayashi, M., Aida, K., Hanyu, I., 1988. Hormone changes during the ovulatory cycle in goldfish. General and Comparative Endocrinology, 69, 301–307.
[30] Lee, C.S., Tamaru, C.S., Weber, G.M., 1987. Studies on the maturation and spawning of milkfish Chanos chanos Forsskal in a photoperiod-controlled room. Journal of the World Aquaculture Society, 18, 253–259.
[31] Pankhurst, N.W., 1997. In vitro steroid production by isolated ovarian follicles of the striped trumpeter. Journal of Fish Biology, 51, 669–685.
[32] Cecconi, S., Rucci, N., Scaldaferri, M.L., Masciulli, M.P., Rossi, G., Moretti, C., D’Armiento, M., Ulisse, S., 1999. Thyroid hormone effects on mouse oocyte maturation and granulosa cell aromatase activity. Endocrinology, 140, 1783–1788.
[33] Gregoraszczuk, E.L., Slomczynska, M., Wilk, R., 2009. Thyroid hormone inhibits aromatase activity in porcine thecal cells cultured alone and in coculture with granulosa cells. Thyroid, 8, 1157–1163.
[34] Sechman, A., Pawlowska, K., Rzasa, J., 2009. Influence of triiodothyronine (T3) on secretion of steroids and thyroid hormone receptor expression in chicken ovarian follicles. Domestic Animal Endocrinology, 37, 61–73.
[35] Milla, S., Wang, N., Mandiki, S.N.M., Kestemont, P., 2009. Corticosteroids: friends or foes of teleost fish reproduction?. Comparative Biochemistry and Physiology, 153A, 242–251.
[36] Haddy, J.A., Pankhurst, N.W., 1999. Stress-induced changes in concentrations of plasma sex steroids in black bream. Journal of Fish Biology, 55, 1304–1316.
[37] Fuzzen, M.L., Bernier, N.J., Van Der, K.G., 2011. Stress and Reproduction. In: Norris, D., Lopez, K. (Eds.), Hormones and Reproduction of Vertebrates. Academic Press, United States. pp. 65–82.
[38] Pottinger, T.G., Carrucj, T.R., 2000. Contrasting seasonal modulation of the stress response in male and female rainbow trout. Journal of Fish Biology, 56, 667–675.
[39] Endo, H., Wu, H., 2019. Biosensors for the assessment of fish health: a review. Fisheries Science, 85, 641–654.
[40] Misra, S., Sahu, N.P., Pal, A.K., Xavier, B., Kumar, S., Mukherjee, S.C., 2006. Pre- and post-challenge immuno-haematological changes in Labeo rohita juveniles fed gelatinised or non-gelatinised carbohydrate with n-3 PUFA. Fish and Shellfish Immunology, 21, 346–356.
[41] Ahmed, I., Sheikh, Z.A., 2019. Hematological and serum biochemical parameters of five freshwater snow trout fish species from river Jhelum of Kashmir Himalaya, India. Comparative Clinical Pathology, 28, 771–782.
[42] Svoboda, M., Kouřil, J., Hamáčková, J., Kalab, P., Savina, L., Svobodova, Z., Vykusova, B., 2001. Biochemical profile of blood plasma of tench (Tinca tinca L.) during pre-and postspawning period. Acta Veterinaria, 70, 259–268.
[43] Kouřil, J., Svoboda, M., Hamackova, J., Kalab, P., Kolarova, J., Lepicova, A., Sedova, M., Savina, L., Moreno Rendón, P., Svobodova, Z., Barth, T., 2007. Repeated administration of different hormonal preparations for artificial propagation and their effects on reproduction, survival and blood biochemistry profiles of female tench (Tinca tinca L.). Czech Journal of Animal Science, 52, 183–188.
[44] Shokr, E.S.A.M., 2015. Effect of follicular stimulating hormone and leutinizing hormone on reproduction, physiological and biochemical changes of Oreochromis niloticus. Egyptian Academic Journal of Biological Sciences, 7, 61–73.
[45] Metwally, M., Veter, I.F.G., 2008. Some biochemical changes associated with injection of grass carp (Ctenopharyngodon idellus) with Oviaprim and Pregnyl for induction of artificial spawning. Global Veterinaria, 2, 320–326.
[46] Ejraei, F., Ghiasi, M., Khara., H., 2015. Evaluation of hematological and plasma indices in grass carp, Ctenopharyngodon idella, with reference to age, sex, and hormonal treatment. Fisheries and Aquatic Life, 23, 163–170.
[47] Falahatkar, B., Poursaeid, S., 2013. Effects of hormonal manipulation on stress responses in male and female broodstocks of pikeperch Sander lucioperca. Aquaculture International, 22, 235–244.
[48] National Research Council., 2011. Guide for the Care and Use of Laboratory Animals: Eighth Edition. The National Academies Press, Washington, p. 246.