Volume 7, Issue 2 (2018)
JFST 2018, 7(2): 145-155 |
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Ghelichi S, Shabanpour B, Pourashouri P. Proximate and Amino Acid Composition, Antioxidant Properties, ACE Inhibitory Effect, and Antibacterial Power of Protein Hydrolysates of Common Carp Roe by Alcalase. JFST 2018; 7 (2) :145-155
URL: http://jfst.modares.ac.ir/article-6-14589-en.html
URL: http://jfst.modares.ac.ir/article-6-14589-en.html
1- Seafood Science & Technology Department, Fisheries & Environmental Science Faculty, Gorgan University of Agricultural Sciences & Natural Resources, Gorgan, Iran
2- Seafood Science & Technology Department, Fisheries & Environmental Science Faculty, Gorgan University of Agricultural Sciences & Natural Resources, Gorgan, Iran , bshabanpour@yahoo.com
2- Seafood Science & Technology Department, Fisheries & Environmental Science Faculty, Gorgan University of Agricultural Sciences & Natural Resources, Gorgan, Iran , bshabanpour@yahoo.com
Abstract: (8595 Views)
Aims: Fish egg has a high nutritional value and is known as an offshore product in the fish processing industry in Asia. Thus, the present study aimed at evaluating proximate and amino acid composition, antioxidant properties, ACE inhibitory effect, and antibacterial power of protein hydrolysates from lyophilized common carp roe by Alcalase.
Materials and Methods: In the present experimental study, the lyophilized roe was subjected to hydrolysis by Alcalase for 30, 60, 90, and 120 minutes at pH 8 and 55˚C. Fat, as well as moisture, ash and protein were measured by AOAC and amino acid composition by high-performance liquid chromatography-mass spectrometry. DPPH scavenging activity, metal ion chelating, ACE inhibitory effect and anti-bacterial power of the samples were analyzed. The data were analyzed by SPSS 21, using ANOVA and LSD tests. Antioxidant property of hydrolysates increased with their concentrations.
Findings: By increasing the hydrolysis time, protein content, protein recovery percentage, and ash content increased, but the amount of fat and moisture decreased. The most abundant amino acids in common carp roe and its protein hydrolysates were valine, lysine, arginine, and leucine. The antioxidant properties of the samples increased with increasing concentration. DPPH scavenging activity of hydrolysates at 20mg/ml was significantly higher than that of BHT solution (p<0.05). All the hydrolysates exhibited antioxidant, ACE inhibitory and antibacterial effects.
Conclusion: Due to the high content of protein, bioactive peptides, and essential and unnecessary amino acids, Common carp roe protein hydrolysates has a high antioxidant property, ACE inhibitory and antibacterial properties.
Materials and Methods: In the present experimental study, the lyophilized roe was subjected to hydrolysis by Alcalase for 30, 60, 90, and 120 minutes at pH 8 and 55˚C. Fat, as well as moisture, ash and protein were measured by AOAC and amino acid composition by high-performance liquid chromatography-mass spectrometry. DPPH scavenging activity, metal ion chelating, ACE inhibitory effect and anti-bacterial power of the samples were analyzed. The data were analyzed by SPSS 21, using ANOVA and LSD tests. Antioxidant property of hydrolysates increased with their concentrations.
Findings: By increasing the hydrolysis time, protein content, protein recovery percentage, and ash content increased, but the amount of fat and moisture decreased. The most abundant amino acids in common carp roe and its protein hydrolysates were valine, lysine, arginine, and leucine. The antioxidant properties of the samples increased with increasing concentration. DPPH scavenging activity of hydrolysates at 20mg/ml was significantly higher than that of BHT solution (p<0.05). All the hydrolysates exhibited antioxidant, ACE inhibitory and antibacterial effects.
Conclusion: Due to the high content of protein, bioactive peptides, and essential and unnecessary amino acids, Common carp roe protein hydrolysates has a high antioxidant property, ACE inhibitory and antibacterial properties.
Keywords: Protein Hydrolysate, Common Carp Roe, Antioxidant Properties, ACE inhibitory Effect, Anti-Bacterial Power
Article Type: Research Article |
Subject:
fish and shellfish physiology
Received: 2017/05/3 | Published: 2018/08/14
Received: 2017/05/3 | Published: 2018/08/14
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